Anion determination

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Anion-exchange chromatographic determination of bromide in serum.

In this liquid-chromatographic method for the determination of bromide in human serum, an important and unique feature is pre-analysis ultrafiltration of the sample, which effectively removes proteins. The chromatographic system consists of an anion-exchange column (Whatman Partisil SAX 10-micron particles), an isocratic phosphate-buffered mobile phase, and ultraviolet detection at 195 nm. The ...

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Anion/anion exchange in human neutrophils

Of the total one-way chloride fluxes (approximately 1.4 meq/liter cell water X min) in steady state human polymorphonuclear leukocytes bathed in 148 mM Cl media, approximately 70% behaves as self-exchange mediated by a nonselective anion carrier that is not inhibited by stilbene disulfonates. Five properties of this carrier-mediated exchange were investigated: substrate saturation is seen with ...

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Anion Analysis 1 - Simultaneous Determination of Chloride and Bromide by a Fluorescence Quenching Method

Introduction Fluorescence spectrophotometry in its pure form is inherently a very sensitive analytical method. Single molecules have been observed in fluorescence microscopy due to the very low signal in the absence of fluorescent analytes (often referred to as zero background) and the possibility of producing many photons from a single molecule using a very bright excitation source. There are ...

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Onboard determination of submicromolar nitrate in seawater by anion-exchange chromatography with lithium chloride eluent.

Ion-exchange chromatography using a high-capacity anion exchanger with UV detection was applied to the determination of nitrate in seawater. Major ions in seawater samples did not affect the peak shape and the retention time of the nitrate when an alkaline metal cation-chloride solution was used as an eluent at high concentrations (0.5-2 mol/l). At a wavelength of 220 nm, the peak of bromide wa...

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Determination of maltitol, isomaltitol, and lactitol by high-pH anion-exchange chromatography with pulsed amperometric detection.

Disaccharide alditols (DAs) such as maltitol, isomaltitol, and lactitol are increasingly being employed in food industry by virtue of their low hydroscopicity, high stability, and good bulking properties. Still, these compounds are reduced-calorie sweeteners, so they are successfully employed in many dietetic foods, like candies, chocolates, baked products, ice creams, and beverages. Here we de...

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ژورنال

عنوان ژورنال: Nature

سال: 1980

ISSN: 0028-0836,1476-4687

DOI: 10.1038/284648b0